Food-borne bacterial pathogens remain a major public health concern, causing extensive illness and economic losses worldwide. Conventional detection methods are often slow and insufficient for identifying viable but non-culturable pathogens. Recent microbiological, biotechnological and bioinformatic advances have markedly improved food safety monitoring. Rapid molecular assays (PCR, qPCR, microarrays), next-generation sequencing, metagenomics, and emerg ing CRISPR-based diagnostics enable faster and more accurate pathogen detection and outbreak tracing. Bioinformatic tools—including genomic databases, phylogenetics, and machine-learning models—support predictive risk assessment and real-time surveillance. Preventive innovations such as bacteriophages, probiotics, antimicrobial pe ptides, nanotechnologybased interventions, and engineered microbes provide sustainable alternatives to chemical preservatives. Key challenges include variability across food matrices, biosafety considerations, and limited integration of multi-omics approaches into routine workflows. Overall, these emerging strategies offer improved precision and responsiveness for detecting and pre venting food-borne bacterial pathogens. Received: 6 November 2025 / Revised: 12 December 2025 / Accepted: 17 December 2025 / Published online: 12 January 2026 © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025 Food-borne bacterial pathogens: emerging approaches in detection and prevention Zaryab Shafi1 · Mohammad Shahid2 · Rahul Singh3 1 3 Archives of Microbiology (2026) 208:109
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Foodborne pathogenic bacteria are one of the main factors causing food safety issues. The rapid and accurate detection of pathogenic bacteria using molecular techniques is an effective and powerful strategy for preventing and controlling outbreaks of foodborne diseases, thereby ensuring food safety. This article summarizes the rapid and efficient molecular diagnostic techniques for detecting pathogenic bacteria, including polymerase chain reaction and its derivatives, isothermal amplification, DNA hybridization, genomic sequencing, and Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-associated (CRISPR/Cas)-based detection technique. Through a comparative analysis of the technical principles, advantages, and potential limitations of these diagnostic methods, as well as an outlook on the future devel opment directions for molecular biological detection technology, which will provide a valuable reference for developing more accurate, convenient, and sensitive methods for foodborne pathogens detection, and will help better address the challenges posed by foodborne diseases, thereby ensuring public health and safety.
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This study aimed to compare the biological performance of a hydraulic calcium silicate cement (EndocemMTA Premixed) containing dimethyl sulphoxide (DMSO), developed to improve handling properties, in comparison with ProRoot mineral trioxide aggregate and Biodentine. Human dental pulp cells were used for in vitro evaluation of cytocompatibility, cell migration and cell–material interactions. Odontogenic differentiation was assessed using a 3D culture model designed to simulate the clinical environment. Pulp capping was performed in vivo on rat maxillary molars, and reparative dentin formation and pulpal inflammatory responses were evaluated using micro-computed tomography (μCT) and histological analyses. In vitro, all investigated materials exhibited comparabl e cytocompatibility, cell migratory behaviour and odontogenic marker expression, with no significant differences among study groups. The μCT analysis demonstrated significantly greater reparative dentin formation in all experimental groups compared with the control, with Biodentine producing a higher dentin volume than EndocemMTA Premixed. Histological evaluation revealed no significant differences among the experimental groups with respect to pulpal inflammation or dentin bridge continuity. These findings suggest that the incorporation of DMSO into premixed formulations would not adversely affect cytocompatibility or pulp healing. Moreover, the 3D model used in this study might serve as a clinically releva
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While there has been a good deal of recent research about teaching transgender singers, less has been written specifically about gender non-binary singers. When the author was approached by a student who shared that they were gender non-binary, the author exam ined her pedagogy to make the classroom a more inclusive space. Using a Performance as Research methodology, the author sur veyed and recorded verbal interviews with her students and dis covered that Estill Voice Training (EVT), the foundation of the voice training in her classes, is built to support inclusivity. In this article, the author, an Estill Master Trainer, shares her journey of working with non-binary students. Definitions and vocabulary of gender identity are discussed and the ways in which EVT supports inclusion in the voice studio ar e explored. At its core, EVT is free of aesthetic bias and aims to train all voices to sing in all voice qualities, supporting students’ identities and natural singing voices. By focus ing on anatomy, EVT uses gender bias-free terminology and offers unlimited vocal options. In addition to detailing the ways in which EVT is an ideal model for teaching gender non-binary singers, the author offers other recommendations for fostering an inclusive environment in the classroom. KEYWORDS Voice; singing; Estill; gender; non-binary; pronouns; inclusion Introduction “Everyone has a beautiful voice.” This quote by Jo Estill, founder of Estill Voice Training®, inspi
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